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Kannan, Dheeban Chakravarthi
- Growth, Lipid Productivity and Cellular Mechanism of Lipid Accumulation in Microalgae Monoraphidium sp. Following Different Phosphorous Concentrations for Biofuel Production
Authors
1 Bioresources and Biotechnology, TERI University, 10 Institutional Area, Vasant Kunj, New Delhi 110 070, IN
2 Biotechnology and Management of Bioresources Division, TERI, India Habitat Centre, Lodhi Road, New Delhi 110 003, IN
Source
Current Science, Vol 112, No 03 (2017), Pagination: 539-548Abstract
Stress to an algal species can be caused by several factors ranging from temperature extremes to varying light intensities under which they accumulate lipids. Stress caused by nutrient limitation is known to induce maximum lipid accumulation in microalgae. In order to obtain an appropriate phosphate concentration exhibiting both high biomass and high lipid contents, four different concentrations of phosphate were implemented. Mechanism of lipid accumulation was also studied. Of the tested concentrations, no significant difference between growth rates was observed. Cultures with phosphate concentration 2.7 mg/l exhibited maximum increase in biomass productivity compared to control. But phosphate concentration 0.5 mg/l demonstrated the highest lipid productivity (8.75 mg/l/day), in accordance with lipid content (21.8%). Further, representation of TEM and fluorescent microscopic images depicted differences in lipid accumulation and sub-cellular ultrastructure at different concentrations of phosphate. Disappearance of phosphate and nitrate from the medium was also evaluated to study the efficient nutrient concentration and to better understand mechanism of lipid enhancement.Keywords
Lipid Accumulation, Monoraphidium sp., Phosphate Concentration, Nutrient Removal Efficiency, Transmission Electron Microscopy.- Optimization Studies for Algae Biofuels Production
Authors
1 Department of Molecular and Cellular Engineering, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad (U.P.), IN
2 The Energy and Resources Institute, New Delhi, IN
Source
Asian Journal of Bio Science, Vol 11, No 1 (2016), Pagination: 42-48Abstract
Continuous use of fossil fuel is globally considered as unsustainable because of depleting supplies and these fuels also account for accumulation of green house gases in the environment. Renewable, carbon neutral, transport fuels are necessary for environmental and economic sustainability. Microalgae feedstocks are gaining interest in the present day energy scenario due to their fast growth potential coupled with relatively high lipid, carbohydrate and nutrients contents. All of these properties render them an excellent source for biofuels such as biodiesel, bioethanol and biomethane; as well as a number of other valuable pharmaceutical and nutraceutical products. It’s a carbon neutral fuel. But high production cost is still a big hurdle in its commercialisation. Various optimisations are discussed in this research for the commercialization of algal biofuels. The production cost of algal biofuel is still quite high, so a lot of optimisation studies for growth parameters, lipid productivity and lipid extraction process are needed, which are discussed in this study. Commercialization of microalgae for biodiesel is technically feasible. Studies had shown that algae biofuel has the potential to completely displace liquid fuels derived from petroleum. Economics of producing microalgal biodiesel need to improve substantially to make it competitive with petrodiesel, but the level of improvement necessary appears to be attainable.